MCAT Question of the Day
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    Q:

    		 Fermentation of recombinant eukaryotic proteins is often done in prokaryotes. Which of the following is not a problem or risk associated with using prokaryotic systems for expression of eukaryotic proteins?
    		 

    A

    		 Prokaryotes do not post-translationally modify their proteins and cannot perform glycosylation or specialized folding tasks.

    B

    		 Prokaryotes contain endotoxin which must be extracted thoroughly before it can be used for human applications.

    C

    		 Prokaryotes may not use the same codons as found on the eukaryotic DNA and thus may not have certain tRNAs, resulting in premature termination of translation.

    D

    		 Prokaryotes have a high frequency of integrating DNA into their chromosomes which blocks transcription almost entirely.



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