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MCAT Question of the Day
Q:
Which of the following is true regarding using dideoxynucleotides (ddNTPs) for DNA sequencing?
A
Restriction enzymes cut the target DNA into fragments, which are then separated and visualized with electrophoresis
B
The template strand is tagged with a radioactive isotope
C
ddATPs, ddCTPs, ddGTPs, and ddTTPs must be utilized in a separate environment from the dATPs, dCTPs, dGTPs, and dTTPs
D
A ddTTP can randomly and occasionally terminate the synthesis of DNA where an adenine occurs in the template strands
Recombinant DNA and Biotechnology
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Nucleic Acid Structure and Function
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DNA Replication and Repair
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